Fig. 5 | Nature Communications

Fig. 5

From: Balancing mcr-1 expression and bacterial survival is a delicate equilibrium between essential cellular defence mechanisms

Fig. 5

The toxic effects of mcr-1 mutants on cell viability. a–c Confocal laser scanning microscopy images of cells treated with l-arabinose (0.2% w/v; 8 h) and stained with LIVE/DEAD® (n = 4). Live and dead cells presented in green and red colour, respectively. d and e Confocal laser scanning microscopy images of control cells treated without l-arabinose for 8 h and stained with LIVE/DEAD® (n = 4). Live and dead cells presented in green and red colour, respectively. f Ratio of dead to live bacteria (biomass) obtained from CLSM z-stack images through COMSTAT analysis (n = 4). The COMSTAT data was assessed using one-way analysis of variance (ANOVA) followed by Tukey–Kramer multiple comparisons post hoc test, '+' and '−' indicate bacteria treated with or without l-arabinose, respectively

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