Fig. 5
From: Rhythmic potassium transport regulates the circadian clock in human red blood cells
RBC circadian rhythms are affected by perturbation of K+ transport. The data points report DEP measurements of G eff (a), and σ cyt (b), in isolated RBCs over 2 days in the presence of 30 nM valinomycin or vehicle control (mean ± SEM, n = 4 biological replicates, cosine fit p value reported). c–e Valinomycin treatment had significant effects on baseline G eff and σ cyt as well as lengthening circadian period of oscillation measured for both DEP parameters, without affecting the amplitude of their oscillation; two-way ANOVA p values (p TWA) and F statistics for interaction or drug effect are indicated, as well as selected Fisher’s post-test p values. f, g The data points report DEP measurements of G eff and σ cyt in isolated RBCs over 2 days in the presence of 11 nM senicapoc, 15 mM TEA or vehicle control, mean ± SEM, n = 4 biological replicates. P values in graphs a, b, f and g indicate report the probability that data series are better described by a damped cosine model than the null hypothesis (straight line); for p > 0.05 the straight line fit is shown, for p < 0.05 the damped cosine fit is shown (solid line)
