Fig. 3
From: 3D microniches reveal the importance of cell size and shape
Focal adhesions formation and cell tension in a 3D microniche. a Representative images of vinculin staining for single hMSCs cultured in 3D microniches with different volumes and geometries. b Fluorescent heat maps of ≥20 cells with the same volume (V 3) but different geometries stained for vinculin. c Representative images of myosin IIa in cells of same geometry but different volumes. d Myosin IIa levels (per cell) as a function of cell volume. e Representative images of cells with different geometries but same volume (V 3). f Myosin IIa levels as a function of cell shape (cylinder and triangular prism) or aspect ratio (cubic and cuboid). g Representative images of myosin IIa and F-actin before and after cells with V 3 treated with 50 μM Blebbistatin (Bleb); bar graph shows quantitation of the changes in the level of myosin IIa after treatment with 50 μM Blebbistatin (Bleb). Data are shown as mean ± s.d. for all panels; n = 45–60 cells analyzed for each data point and *P < 0.05, **P < 0.01 (ANOVA using a Tukey post-test). Scale bar for all images is 20 μm
