Fig. 4

Nuclear function and transcription factor activity a Nucleus volume and height as a function of cell volume. The volume of nucleus was calculated by fitting the morphology of nucleus to an ellipsoidal shape. Data are given as mean ± s.d. with 12 ≤ n ≤ 15. b Quantitation of nucleus average spatial density (total DAPI intensity per nuclear volume) as a function of cell volume. Highly condensed domains show higher fluorescence intensity. The scale bar is 5 μm. c Quantitation of the changes in the level of nucleus average spatial density for cells with V ₃ after treatment with 1 μM cytochalasin D (Cyto D) or 50 μM Blebbistatin (Bleb). d Representative images and quantification of YAP/TAZ localization in hMSCs with different cell volumes but same geometry after 24 h. Scale bar 10 µm. e Representative images and quantification of YAP/TAZ localization in hMSCs with different cell geometries but same volume after 24 h. Scale bar 10 µm. f Representative images of hMSCs stained for HDAC3 on cells with different volumes but same geometry. Histogram shows nuclear HDAC3 levels as a function of cell volume. g Representative images and quantitation of cells with V ₃ treated with 50 μM Blebbistatin (Bleb). Data are shown as mean ± s.d. for all panels, n = 50–60 cells analyzed for each data point. *P < 0.05, **P < 0.01 (ANOVA using a Tukey post-test). NS no significant difference