Fig. 6
From: 3D microniches reveal the importance of cell size and shape
Size and geometry affect single hMSC fate. a Alkaline phosphatase (ALP) staining for cells with different V 1 and V 3 volume. The ALP-positive cells were determined by applying an optimal threshold to the image; ALP intensity above the threshold was determined as ALP positive. b Quantification of differentiation after 7 days (ALP) and 10 days (Oil Red O) for cells with different volumes. c Representative images show Oil Red O positive and negative staining for cells with volume (V 3) but different geometries. d Quantification of adipogenic differentiation after 10 days for cells with different geometries. Mean ± s.d., ANOVA one-way analysis followed by Tukey post-hoc test shows significance levels of *P < 0.05, **P < 0.01. NS no significant difference. N ≥ 6 regions of interest (ROI) with a total of 150–200 cells analyzed. Scale bar 20 µm
