Table 2 Pre-steady-state kinetic parameters of the WT and mutant APE1 proteins

From: Molecular snapshots of APE1 proofreading mismatches and removing DNA damage

APE1 Variant

[APE1]active (nM)

kobs (s−1)

kss (s−1)

3′ mismatch (exo)

 WT

26 ± 2

0.9 ± 0.2

0.045 ± 0.008

 F266A

25 ± 5

21 ± 7

2.1 ± 0.7

 M270A

22 ± 4

1.9 ± 0.9

0.13 ± 0.03

 R177A

13 ± 1

1.0 ± 0.2

0.14 ± 0.01

 W280A

17 ± 4

0.5 ± 0.2

0.21 ± 0.05

THF (endo)

 WTa

11

36 ± 6

0.8 ± 0.1

  1. a Freudenthal, B. D. et al.11
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