Fig. 5

Effects of FAM46A alterations (knockdown and overexpression) on leptin secretion. a leptin release in the FAM46A knockdown. b leptin release upon overexpression of FAM46A. Nine-day-old differentiating preadipocytes were cultured in serum-free basal medium without insulin or after stimulation with 100 nM insulin for 6 h. Conditioned medium was removed and the secreted leptin content was measured using a DuoSet ELISA kit. Accumulated leptin was normalized to total amount of protein from each well. Results are given as the mean ± s.d. (n = 11) with *p < 0.05, **p < 0.01 for statistical significance calculated using a unilateral Student’s t test for an increased or a decreased leptin release according to a knockdown or an overexpression of FAM46A, respectively