Fig. 6

Pharmacological manipulations change the nuclear size of hepatocytes in culture. a Representative photos showing size change of nucleus after the treatment of Mek inhibitor (U0126) or Mkp1 inhibitor (BCI). Nuclei were stained blue with Hoechst-33342 (blue). Counterstained with tubulin (green). b Quantification of the alternation of nuclear size between 0 h or 46 h of treatment. Note that Mek inhibitor (U0126) treatment increases the nuclear sizes of WT hepatocytes to those in polyploidy Per-null mice. In contrast, Mkp1 inhibitor (BCI) reduced the nuclear sizes of Per-null hepatocytes to those in WT mice. c Frequency distribution of nuclear size of hepatocytes after 0 h and 46 h drug treatment. Data are representative of at least three independent experiments. Values are the mean ± SEM by Two-way ANOVA with Bonferroni’s post-test., n > 3000 cells (three experiments). *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar, 100 μm