Fig. 1
From: L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast
Schematic overview of L-SCRaMbLE. a Mode of action in cells harboring pLH_Scr15. In darkness or far-red light (λ = 740 nm), PIF3-CreC is located in the nucleus due to an incorporated NLS, while PhyBNT-CreN is located in the cytoplasm. Upon red light illumination (λ = 660 nm) PhyBNT-CreN binds PIF3-CreC in the cytoplasm (not shown), thereby reconstituting Cre recombinase from its split halves. Reconstituted Cre moves to the nucleus where it induces recombination between the loxPsym sites. b Mode of action in cells harboring pLH_Scr16. In darkness or far-red light (λ = 740 nm) PIF3-CreC and PhyBNT-CreN are separately located in the nucleus. Upon red light illumination (λ = 660 nm) PhyBNT-CreN binds PIF3-CreC, thereby reconstituting Cre recombinase from its split parts, allowing recombination at loxPsym sites
