Fig. 6

Morphological changes of hippocampal tissue specimen after AP-nanoPALDI process. a–e Coherent anti-stokes Raman scattering (CARS) images taken with regards to the vibrational modes from 2750 to 2950 cm⁻¹ after AP-nanoPALDI MS analysis; a top view, b side view of the orange rectangle in a, and c zoomed-in view of the magenta square in a. d The CARS intensity profile along the double sided arrows in c, shows the length of one trench was very close to 5 µm. e Cross-sectional view across the horizontally drawn blue dotted line in a. f Line patterns profile by 3D confocal imaging and, g helium ion microscopy (HIM) imaging of individual line patterns ablated by AP-nanoPALDI. All the images were taken after the hippocampal tissue was dried. Scale bars, a 100 µm, c 50 µm, and g 50 μm