Table 1 Assigned lipids, metabolites, and derivatives from mouse hippocampal tissue slices in the positive ion mode using AP-nanoPALDI MS

Compound	Measured m/z	Theoretical m/z	Error (ppm)	Molecular formula	Species	Ref.
Sterol lipid
Cholesterol	385.3459	385.3464	1.30	C₂₇H₄₅O	[M − H]⁺	³⁷
	369.3521	369.3515	−1.62	C₂₇H₄₅	[M + H − H₂O]⁺	³⁹
Glycerolipid
MAG 16:1	311.2575	311.2581	1.93	C₁₉H₃₅O₃	[M + H − H₂O]⁺	³⁶
	329.2692	329.2686	−1.82	C₁₉H₃₇O₄	[M + H]⁺	⁷¹
MAG 16:0	313.2731	313.2737	1.92	C₁₉H₃₇O₃	[M + H − H₂O]⁺	³⁶
	331.2851	331.2843	−2.41	C₁₉H₃₉O₄	[M + H]⁺	⁷¹
MAG 18:2	337.2732	337.2737	1.48	C₂₁H₃₇O₃	[M + H − H₂O]⁺	³⁶
	355.2851	355.2843	−2.25	C₂₁H₃₉O₄	[M + H]⁺	⁷¹
MAG 18:1	339.2889	339.2894	1.47	C₂₁H₃₉O₃	[M + H − H₂O]⁺	³⁶
	357.3007	357.2999	−2.24	C₂₁H₄₁O₄	[M + H]⁺	⁷¹
MAG 18:0	341.3046	341.3050	1.17	C₂₁H₄₁O₃	[M + H − H₂O]⁺	³⁶
	359.3161	359.3156	−1.39	C₂₁H₄₃O₄	[[M + H]⁺	⁷¹
Sphingolipid
Ceramide 18:0	548.5390	548.5401	2.01	C₃₆H₇₀NO₂	[M + H − H₂O]⁺	⁷²
Sphingosine	282.2785	282.2791	2.13	C₁₈H₃₆NO	[M + H − H₂O]⁺	⁷³
	300.2905	300.2897	−2.66	C₁₈H₃₈NO₂	[M + H]⁺	⁷³
Sphinganine	284.2945	284.2947	0.70	C₁₈H₃₈NO	[M + H − H₂O]⁺	⁷³
	302.3062	302.3054	−2.65	C₁₈H₄₀NO₂	[M + H]⁺	⁷³
Adenine	136.0614	136.0617	2.20	C₅H₆N₅	[M + H]⁺	⁵³
Phosphocholine	184.0754	184.0733	−11.41	C₅H₁₅NO₄P	[M]⁺	⁴⁰

The table includes exact mass measurements of ion peaks in the mouse hippocampal tissue slice by AP-nanoPALDI-QE-Orbitrap (number of C atoms: degree of unsaturation)

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