Fig. 3
Detection of Zika vRNA in tissues in subcutaneously inoculated animals or animals challenged via mosquito bite. qRT–PCR was used to assess the Zika viral burden and tissue distribution in subcutaneously inoculated animals vs. animals challenged via mosquito bite. Orange symbols represent animals infected via subcutaneous injection and blue symbols represent animals that were infected via exposure to ZIKV-infected Aedes aegypti. Approximately 24 different tissues were assessed for the presence of viral RNA. Shown are the tissues with positive detection in at least one of the animals per group. The qRT–PCR assay has a quantification threshold of three copies/reaction
