Fig. 4
Single-nucleotide polymorphisms in ZIKV populations infecting monkeys and mosquitoes in this study. We used Illumina deep sequencing to characterize viral genomic diversity in a saliva samples from mosquitoes that fed on ZIKV-infected Ifnar−/− mice, b monkeys infected via mosquito bite, and c monkeys inoculated subcutaneously with ZIKV-PRVABC59 stock. Purple symbols represent individual saliva samples, blue symbols represent animals that were infected via exposure to ZIKV-infected Aedes aegypti, and orange symbols represent animals infected via subcutaneous injection. Frequencies of SNPs detected in the stock virus isolate are plotted in each panel for reference. Viruses infecting monkeys were sequenced at the time of peak plasma viremia: day 3 post-infection in sc-inoculated animals and days 5 or 6 post-feeding in mosquito-bitten animals. Plotted frequencies represent the average of 2 technical replicates for each sample
