Fig. 1

Pof8 structural analysis reveals a La motif and two RRM domains. a The domain architecture of S. pombe (Sp) Pof8 compared to T. thermophila (Tt) telomerase subunit p65, E. aediculatus (Ea) telomerase subunit p43, Homo sapiens (Hs) LARP7 and LARP3, and the S. pombe La protein, Sla1. Numbers above the Pof8 sequence indicate predicted domain boundaries. b Alignment and secondary structure elements of the C-terminal RRM domains based on HHPred predictions; beta strands are highlighted with blue arrows, and alpha helices are highlighted in red. The secondary structure elements for p65 are based on the crystal structure²⁷ and are labeled above the alignment. Forty amino acids that form an extended loop between β2 and β3 in p65 have been abbreviated -40- to keep the alignment compact. c N-terminal La motif and RRM1 domains. Residues shown to interact with oligo-uridine RNA are boxed and are mostly not conserved in Pof8. The p65 secondary structure elements are labeled above the alignment