Fig. 8
From: The podoplanin-CLEC-2 axis inhibits inflammation in sepsis
Podoplanin-deficient hematopoietic cells alter macrophage migration to the infected peritoneum. PDPNfl/flVAVcre+ or littermate control mice were subjected to cecal ligation and puncture (CLP) for 24 h. Immune cell recruitment into the peritoneal cavity was assessed by flow cytometry. a Percentage and b total number of myeloid cells (CD45+CD11b+) in peritoneal lavage fluid (PLF). c Percentage and d total number of neutrophils (CD45+CD11b+Ly6G+) in PLF. e Percentage and f total number of macrophages (CD45+CD11b+F4/80+) in PLF. g Percentage of macrophages expressing podoplanin. h Correlation between percentage of macrophages recruited and clinical score. i TNF-α, j CCL-2, k CXCL-1, and l IL-10 levels detected in the PLF. Data are means ± s.d. (7 mice in CLP groups, n = 4 in sham group). Differences assessed using Mann–Whitney U-test. *p < 0.05, **p < 0.01, ****p < 0.0001
