Fig. 5
Brain activity pattern screening substantially reduces the false-positive rate. (Left) All compounds are ranked based on composite LFP scores. Compounds with a wild-type activity profile based on deep behavioral phenotyping (right column) are indicated in cyan; those with an abnormal profile are indicated in magenta. Top hits based on composite LFP scores are indicated in the blue box. (Right) Classification based on deep behavioral phenotyping (n = 10+ larvae, each subjected to four independent light stimuli) of all hits from the preliminary (single-metric) behavioral screen (n = 8+ larvae, each subjected to four independent light stimuli) and the LFP-complexity screen (n = 5–11 per compound). 26 out of 31 hits (83.9%) identified in the preliminary behavioral screen exhibit significant behavioral abnormalities when evaluated in detail. In contrast, only 1 out of 6 hits (16.7%) based on the composite LFP score has similar behavioral abnormalities
