Fig. 4 | Nature Communications

Fig. 4

From: Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions

Fig. 4

GECX-MS to capture and identify Trx-interacting proteins in E. coli. a Schematic illustration of disulfide reduction by thioredoxin (Trx). b Western blot of cell lysate of E. coli cells expressing Trx1(32BprY-33S-36A), showing multiple endogenous proteins crosslinked to Trx1. c List of proteins crosslinked to Trx1(32BprY-33S-36A) in E. coli cells that have the crosslinked peptides identified. The crosslinked Cys residues are highlighted in red. d Mass spectrum of cross-linked peptide between iscU and Trx1. U represents BprY in the peptide sequence. The mass spectra for other crosslinked proteins are provided in Supplementary Figure 9

Back to article page