Fig. 1

Pml regulates MSCs cell autonomously. a Pml expression in MSCs derived from Pml ^+/+ or Pml ^−/− mice is shown on the left. Representative plots in the middle show the percentage of MSCs in Pml ^+/+ or Pml ^−/− mice, while the chart on the right shows the relative numbers of MSCs, and stromal cells in Pml ^−/− mice compared to Pml ^+/+ mice (n = 7). b Percentage and numbers of MSCs in Pml ^+/+ or Pml ^−/− mice of 15 months of age. The chart on the right shows the relative numbers of MSCs, and stromal cells in Pml ^−/− mice compared to Pml ^+/+ mice (n = 6). c Pml expression in MSCs derived from Prrx1-Cre-Pml ^+/+ or Prrx1-Cre-Pml ^F/F mice is shown on the left of each panel. Representative plots in the middle show the percentage of MSCs in Prrx1-Cre-Pml ^+/+ or Prrx1-Cre-Pml ^F/F mice, while the chart on the right shows the relative numbers of MSCs, and stromal cells in Prrx1-Cre-Pml ^F/F mice compared to Prrx1-Cre-Pml^+/+ mice (n = 6). d CFU-F colony forming capacity of MSCs derived from Pml ^+/+ or Pml ^−/− mice after 5 days in hypoxic conditions. The quantification of the colonies is shown on the left, while representative pictures of the colonies stained with crystal violet, and of the morphology of the cells are shown on the right (n = 7). e Spontaneous differentiation of Pml ^+/+ and Pml ^−/− MSCs. The expression levels of Pparg, Fabp4, and c/ebpb have been analyzed to determine the spontaneous differentiation of the cells to adipocytes; while the expression levels of Runx2, Sp7, and Alp have been used to investigate the capacity of the cells to differentiate toward osteoblasts. The charts show biological replicates ± SEM. f Capacity of Pml ^+/+ and Pml ^−/− MSCs to differentiate into adipocytes and into osteoblasts once triggered with specific factors. The chart on the left shows the relative expression of Fabp4 in MSCs Pml ^+/+ or Pml ^−/−, cultured in hypoxic conditions for 5 days, and then subjected (or not) to adipogenesis. (A. indicates cells induced to differentiate into adipocytes). The chart on the right shows the relative expression of Alp mRNA in MSCs induced to differentiate to osteoblasts (O indicates cells induced to differentiate into osteoblasts). The charts show one representative experiment, n = 2