Fig. 7
From: The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading
Pex15 bridges Pex5/Pex14 with Pex1/Pex6. a A pull-down binding assay of GST-Pex14 with Pex5, Pex15 1-327-FLAG, and Pex1/Pex6 in the presence of ATP shows that Pex1/Pex6 associates indirectly with Pex14 through an interaction requiring Pex5 and Pex15. Pex15-FLAG was detected by an anti-FLAG western blot on the input and elution. b A pull-down binding assay of MBP-Pex15 1-327 with Pex1/Pex6-WB2 and fluorescein labeled Pex5 with and without a PTS1 peptide. MBP-Pex15 and Pex5 migrate similarly, so the presence of Pex5 was determined by imaging its fluorescein label. MBP alone does not bind Pex5 (Supplementary Figure 9)
