Fig. 5
Plasmodium infection does not change host’s circadian period. a Representative actograms of daily wheel-running activity of control and Plasmodium-infected mice (LD). All running-wheel experiments involve seven days on LD12:12 followed by 10 days in DD to confirm that all animals have a normal circadian rhythm, after which animals are either infected or injected with vehicle. Horizontal black and white bars at the top of each actogram represent lights off and on for the initial acclimatization period, respectively. b Representative actograms of daily wheel-running activity of control and Plasmodium-infected mice in constant darkness (DD). c Total daily and relative rest-period activity levels of control (n = 4) and infected (n = 8) mice considering the whole infection time and a 20-days post-infection interval. d Period of running-wheel activity of control (n = 4) and infected (n = 8) mice. Error bars show mean ± s.e.m.; *p < 0.05, **p < 0.01, ***p < 0.001 tested with Mann–Whitney test in panels c and d. e PER2::LUC period plot of various tissues harvested from control (black) and Plasmodium-infected (blue) mice. No significance was found, p > 0.05, FDR method Benjamini–Hochberg
