Fig. 8
Estimating auto-fluorescence and fluorescence decay. a Estimated auto-fluorescence per micrometre cell length a* and its error bar σ a for 284 cells for which fluorescence was fit as a function of cell size. The red line (a = 433.5) is the fit obtained when all cells are assumed to have a common fluorescence per micrometre a. The green line is obtained with a mixture model that allows for “outliers” from a uniform distribution (a = 421.8). b The joint probability density of a given by the mixture of Gaussian distributions for all 284 cells. c GFP decay (including bleaching) was estimated by fitting observations of decreasing GFP levels during the glucose phases in the switching experiments, when no GFP is synthesized, to an exponential function of time. The panel shows the cumulative distribution of the Pearson correlations between the estimated log-GFP levels and time. d Estimated decay rates for individual cells (vertical axis) as a function of absolute GFP level of the cell (horizontal axis). The dashed line shows the overall estimated rate used in subsequent analysis
