Fig. 4
Comparison between CBR2opt and Luc2 utilizing either D-LH2 or Aka-HCl for imaging mouse brain. a Images of representative mice after intracranial implantation of 1 × 105 HEK-EF1-Luc2-T2A-copGFP or HEK-EF1-CBR2opt-T2A-copGFP cells and intravenous injection of D-LH2 (300 mg kg−1) or NH2-NpLH2 (220 mg kg−1) (n = 4). b Quantification of photon flux (photon per s) with an exposure time of 30 s at the peak of bioluminescence emission (4–7 min after substrate injection) (*p < 0.05; **p < 0.01 compared to CBR2opt/D-LH2). c Images of representative mice after intracranial implantation of cells and intravenous injection of either D-LH2 (300 mg kg−1) or Aka-HCl (50 mg kg−1) (n = 3). d Quantification of flux (photon/s) with an exposure time of 30 s (**p < 0.01 compared to CBR2opt/D-LH2). Error bars represent S.D.
