Fig. 5
Bioluminescence tomography in mouse brain showing the location of the bioluminescence signal in mouse brain at day 1 after transplantation of cells. The images were acquired 10 min after intraperitoneal injection of NH2-NpLH2 (220 mg kg−1) or D-LH2 (300 mg kg−1) using respectively band pass filters 700 nm, 720 nm, and 740 nm or 580 nm, 600 nm, and 620 nm for each mouse. Acquisition time was 30 s. After BLI mice kept in the dedicated coil were placed in the CT scanner. a Co-registered CT and BLT images using CBR2opt/D-LH2 pairing (sagittal section and reconstruction). b Co-registered CT and BLT images using CBR2opt/NH2-NpLH2 pairing (sagittal section and reconstruction). c Quantification of depth from skull surface indicated no significant difference (ns) between substrates when using the single point light source (n = 3). Error bars represent S.D.
