Fig. 6
Bioluminescence tomography in mouse brain showing the location of the bioluminescence signal at day 5 after transplantation of cells. The images were acquired after intraperitoneal injection of NH2-NpLH2 (220 mg kg−1) or D-LH2 (300 mg kg−1) using respectively band pass filters 700 nm, 720 nm, and 740 nm or 580 nm, 600 nm, and 620 nm for each mouse. Acquisition time was 30 s. After BLI mice kept in the dedicated coil were placed in the CT scanner. a Coronal, transaxial and sagittal view of co-registered CT and BLT images using CBR2opt/D-LH2 pairing. b Coronal, transaxial and sagittal view of co-registered CT and BLT images using the CBR2opt/NH2-NpLH2 pair. Migration of cells can be clearly seen at day 5 (two adjacent light sources are represented). c Histological analysis showing the presence of two groups of cells (copGFP) in both ventricular areas of brain (green signal = copGFP; blue = nuclei). Scale bars = 500 μm (left panel) and 10 μm (right panel)
