Table 2 Kinetic parameters for beetle luciferases and substrates

From: Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging

 

Brightnessa (RLUmax µM−1)

Km (µM)

 

D-LH2

NH2-NpLH2

D-LH2

NH2-NpLH2

ATP (saturating D-LH2)

ATP (saturating NH2-NpLH2)

Luc2

3 × 109

*

1

*

286

*

CBR

6 × 108

6 × 105

10

0.3

159

132

CBR2

4 × 108

8 × 104

22

0.5

305

112

  1. * Value could not be determined because of insufficient signal above background at low substrate concentrations
  2. a Brightness (RLUmax µM−1 enzyme) and Km calculated from substrate titrations using Michaelis–Menten regression analysis; n = 3. Brightness values for NH2-NpLH2 were lower than D-LH2 partly because of luminometer PMT bias for shorter wavelength photons
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