Fig. 5
From: Controlling protein activity by dynamic recruitment on a supramolecular polymer platform
Reversible control of enzyme recruitment via DNA strand exchange. a The enzyme is recruited to polymers pre-assembled with inhibitor protein by the addition of recruiter strand RET. A 10-nucleotide overhang (toehold) appended to the recruiter strand (RET) allows its displacement by a fully complementary displacer strand (D) via toehold-mediated strand displacement. b Normalized enzyme activity upon the sequential addition of recruiter and displacer oligonucleotides. Each iteration, a twofold molar excess of either recruiter or displacer strand is added with respect to the previous stage. Enzyme activities were normalized to a control without inhibitor protein. Experiments were performed using 25% BTA-DNA (0.5 μM BTA-DNA, 1.5 μM BTA-3OH), 20 nM RI, 1 nM β-lactamase and 10 nM BLIP, 0 – 340 nM RE and 0-168 nM D. Error bars represent s.d. of triplicate measurements
