Fig. 4
Gross cellular analysis of the MOR174–9 domain. a Typical result of the experimental approach. Left image showing the GFP-positive MOR174–9 glomerulus in a horizontal slice of the olfactory bulb. Right image showing TMR-dextran fluorescence after successful electroporation. Scale bars = 100 µm. Blow-up: overlay of the two channels with marked cell bodies (yellow). Scale bar = 100 µm. b In total, a median of 162 (Q1 +25.75; Q3 −26.75) cells (n = 5 animals) projecting to the electroporated MOR174–9 glomerulus is found. Colored triangles indicate the total number of cells for each individual experiment. c Variability of percentile borders (i.e., distance to the center ± s.d. (µm)) across all experiments as a function of decile. d Relative Euclidean distance distribution of the cells associated with the “standard” MOR174–9 glomerulus (red vertical bars, bin size of 10 µm). Red bars refer to the left Y-axis and show the fraction of cells within each bin and the corresponding SDs among the five experiments (dark blue bars). Dashed black line represents the cumulative plot of the histogram and refers to the right Y-axis. e Illustration indicating the four layers of the bulb, which are considered separately: GL (white), outer quarter of the EPL (magenta), inner three quarters of the EPL (yellow), and MCL (red-cyan). For completeness, olfactory nerve layer (ONL), IPL, and main cell types are also shown. PG = periglomerular cell, eTC = external tufted cell, TC = tufted cell, MC = Mitral cell. f Scatter plot of the distances of all cells (Y-axis) to the glomerular center separated by layer identity of the cell soma localization (X-axis). Same color code of the dots as in b. Numbers in the dashed bubbles indicate mean number (Ø) of cells of each layer per glomerulus. g Pie chart illustrating the average relative cellular composition of the MOR174–9 glomerulus, according to layer identity
