Fig. 3
From: A biosensor-based framework to measure latent proteostasis capacity
Barnase foldedness and aggregation can be readily assessed by flow cytometry. a Flow cytometry analysis to determine FRET. Data points indicate fluorescence signatures of individual cells for donor and FRET channels. b Representative confocal images of cells recovered by cell sorting (scale bar=10 µm; “Soluble” image scaled at 4×brightness vs. “Aggregated”). c Conceptual framework for how flow cytometry data reports on foldedness vs. aggregation d The Lower-slope gradients of barnase mutants measured by flow cytometry (right axis) follows the expected relationship between stability (ΔG F ) and fraction folded (left axis, scaled to fit). Each data point reflects one barnase mutant (mean ± SD; three replicates). Note that the AFU or AU scales, while arbitrary, cannot be compared between different panels and figures due to changes in instrument calibration settings
