Fig. 4
From: A biosensor-based framework to measure latent proteostasis capacity
Quantifying proteostasis by changes in latent chaperone concentration available for holdase activity (ΔC). a Conceptual framework for how chaperone levels affect barnase foldedness. b Changes in Lower-slope gradients of the barnase mutants (data points show individual replicates of each mutant) vs. a FRET-positive negative control (mTFP1 cp175-Venus cp173 fusion lacking the barnase kernel; data points show individual replicates) when co-expressed with HSP40 and HSP70 chaperones (DNAJB1 and HSPA1A respectively) relative to baseline conditions (Y66L EGFP co-expression). Means ± SEM shown. c Effect of toggling proteostasis on changes in available chaperone capacity (ΔC). Plots show means ± SEM of the 12 barnase mutants when coexpressed with DNAJB1 and HSPA1A (compared to Y66L EGFP control; left panel), or treated with proteostasis-modulating drugs (compared to untreated control). Wilcoxon signed rank tests results coded as ***p < 0.001, **p < 0.01, *p < 0.05
