Fig. 2
Exosomal miR-1247-3p is characteristically secreted by high-metastatic liver cancer cells and mediates fibroblasts activation. a Microarray analysis of exosomal miRNAs from different cancer cells were presented in a heatmap. b Overlapping results of upregulated miRNAs in indicated groups. c qRT-PCR analysis of pro-inflammatory genes expression of MRC5 transfected with indicated mimics. d Migration assay of MRC5 transfected miR-1247-mimic or normal control. Migrated cells were counted and representative images were shown. Scale bar, 150 μm. e Migration ability comparison of MRC5 treated with exosomes derived from CSQT-2 or HCC-LM3 with stably expressing miR-1247-3p inhibitor or negative control. Migrated cells were counted and representative images were shown. Scale bar, 150 μm. f qRT-PCR assay of indicated genes expression level of MRC5 treated with exosomes derived from HepG2 versus CSQT-2 or MHCC-97L versus HCC-LM3 in the presence of miR-1247-3p inhibitor or not. Experiments were performed at least in triplicate and results are shown as mean ± s.d. Student’s t-test was used to analyze the data (*p < 0.05; **p < 0.01; ***p < 0.001)
