Fig. 2
From: Gs- versus Golf-dependent functional selectivity mediated by the dopamine D1 receptor
a Homology modeling of Gs (green) coupling to D1R (cyan) based on β2AR-Gs crystal structure (PDB: 3SN6) with the amino acid alignment among Gs (top row), Golf (middle row), and Golf/s_33–38 chimera (bottom row). b Dose-response curves of DHX-induced BRET between D1R-Rluc and Gs-Venus, Golf-Venus, or Golf/s_33–38-Venus (black, orange, and red respectively). BRET values are normalized to Emax values obtained by DA with corresponding Gα-Venus constructs. c, d Close-up views of the simulated interface between D1R and Gs (c) or D1R and Golf (d) at the intracellular loop 2 (IL2) of D1R and N-terminal α-helix (αN) of Gα subunit. e Frequency density distribution plot of 50 ns simulation for D1R-Gs (black) and D1R-Golf (orange). Proportions of salt bridge occurance (<4 Å) in the simulation are shown in percentage. The error bars represent S.E.M.
