Fig. 4
IFNγ increases proangiogenic monocyte adhesion but abolishes transmigration. a Analysis of proangiogenic versus non-angiogenic monocyte adhesion to endothelial monolayer activated with different inflammatory cytokines. n = 4 independent experiments with monocytes from four different donors, cell counted in 1 mm2 area, the data presented as mean ± s.d., *p-value <0.05, **p-value <0.005 in ANOVA test. Proangiogenic monocytes adhered at higher levels to IFNγ-activated endothelium. b Analysis of proangiogenic versus non-angiogenic monocyte transmigration to the endothelial monolayers activated with indicated inflammatory cytokines. n = 4 independent experiments with monocytes from four different donors, 40 individual cells tracked per condition, the data presented as mean ± s.d., *p-value <0.05, **p-value <0.005 in ANOVA test. IFNγ-activated endothelium exhibited the lowest transmigration rate for proangiogenic monocytes. c Analysis of the dose-response effect of IFNγ on monocyte subset adhesion to TNF-activated endothelial monolayer. n = 3 independent experiments with monocytes from three different donors, cell counted in 1 mm2 area, the data presented as mean ± s.d. IFNγ induced a dose-dependent increase in proangiogenic monocyte adhesion to TNF-activated endothelium. d Effect of IFNγ on monocyte transmigration through TNF-activated endothelial monolayers. n = 3 independent experiments with monocytes from three different donors, the data presented as mean ± s.d. Increasing concentrations of IFNγ induced a dose-dependent inhibition of proangiogenic monocyte transmigration through TNF-activated endothelial monolayers
