Fig. 5

Human proangiogenic monocytes transmigrate in angiogenic factor-driven inflammation. a Time-lapse imaging of proangiogenic monocyte transmigration assay under flow through endothelium activated with a combination of TNF and VEGF-A. Scale bar = 25 µm. Green and blue arrows indicate two transmigrating proangiogenic monocytes. b Analysis of proangiogenic and non-angiogenic monocyte adhesion in ADIn (TNF + VEGF-A or TNF + FGF2) versus conventional (TNF alone) inflammation under flow. c Analysis of proangiogenic and non-angiogenic monocyte transmigration in ADIn versus conventional inflammation under flow. The data are presented as mean ± s.d., *p-value <0.05 and **p-value <0.005 in ANOVA test. d Analysis of the directionality of monocyte subsets in ADIn versus conventional inflammation. The data in d are presented as mean ± s.d., * indicates p-value <0.05 in Mann–Whitney test. The data were from 4 independent experiments