Fig. 3

TDRD5 selectively controls the production of piRNA cluster-derived pachytene piRNAs. a Genomic annotation of total piRNA from adult WT, Tdrd5^cKO, and Miwi^KO testes. piRNA clusters: 214 piRNA clusters defined by Li et al.¹⁹. b Relative abundance of total piRNA from adult WT, Tdrd5^cKO, and Miwi^KO testes normalized by miRNA. Note specific reduction of piRNA cluster-derived piRNAs in Tdrd5^cKO testes. c Genomic annotation of MILI-bound piRNAs from adult WT, Tdrd5^cKO, and Miwi^KO testes. d Genomic annotation of MIWI-bound piRNAs from adult WT and Tdrd5^cKO testes. e Genomic annotation of total piRNA from sorted pachytene spermatocytes (PS) from WT and Tdrd5^cKO testes. f Relative abundance of total piRNA from sorted pachytene spermatocytes from WT and Tdrd5^cKO testes normalized by miRNA