Fig. 4

Loss of TDRD5 selectively reduces piRNA production from a subset of top-piRNA-producing loci within 214 piRNA clusters. a Shown is the number of piRNA reads mapped to 214 individual piRNA clusters from small RNA libraries of WT and Tdrd5^cKO testes. piRNA reads were normalized by the miRNA counts of each small RNA library. piRNA clusters are ranked by the their piRNA abundances in WT library. b Comparison of piRNA fold change (Tdrd5^cKO/WT or Miwi^KO/WT) of top150 piRNA-producing clusters from indicated total small RNA libraries. piRNA reads were normalized by miRNA counts of each library. X-axis represents the top150 piRNA clusters ranked by their piRNA abundances in WT library in descending order. c Comparison of piRNA fold change (Tdrd5^cKO/WT or Miwi^KO/WT) of top150 piRNA-producing clusters from indicated MILI-piRNA libraries. piRNA reads were normalized by total reads of each library. X-axis represents the top150 piRNA clusters ranked by their piRNA abundances in WT library in descending order