Fig. 7

TDRD5 directly binds piRNA precursors. a Autoradiography (left) and western blot (right) of TDRD5-RNA and MILI-RNA complexes from CLIPs. IgG served as negative control. Red lines indicate the corresponding RNA regions that were extracted from the membrane. HC, Ig heavy chain; LC, Ig light chain. b Size distribution of RNA reads from TDRD5-CLIP libraries. n = 3; Error bars represent s.e.m. c Size distribution of RNA reads from MILI-CLIP library. d Nucleotide composition of TDRD5-CLIP reads. n = 3; Error bars represent s.e.m. e Nucleotide composition of MILI-CLIP reads. f Genomic annotation of TDRD5-CLIP reads and MILI-CLIP reads. TDRD5-CLIP libraries, n = 3; Error bars represent s.e.m. g Scatter plot of piRNA reads mapped to 214 individual piRNA clusters from TDRD5-CLIP and MILI-CLIP libraries. Pearson correlation (R²) is shown. h Density plots of total piRNA reads and TDRD5-CLIP reads mapped to each of the 100 positions proportionally divided in all 214 piRNA clusters. Each of the 214 piRNA clusters is equally divided into 100 sequence fragments. The total piRNA reads or TDRD5-CLIP reads were mapped to each fragment of each piRNA cluster and total mapped reads for each position were added up for all 214 clusters. Pearson correlation (R²) is shown