Fig. 4
From: Engineering human pluripotent stem cells into a functional skeletal muscle tissue
Contractile properties of hPSC-derived iSKM bundles. a Representative contractile force traces of a 4-week TRiPS-derived bundle shows increase in contractile force with increase in stimulation frequency and formation of tetanic contraction. b Increase of twitch and tetanus (at 40 Hz) force with time of culture. (*P < 0.05 vs. 1 week, Tukey-Kramer HSD test; n = 6–8 bundles per group at each time point). c Specific force and tetanus-to-twitch ratio of 2-week H9 and TRiPS derived bundles (n = 10 bundles for H9, 12 bundles for TRiPSC). d Representative peak GCaMP6 fluorescence images during spontaneous (spon) and electrically stimulated calcium transients in 2-week bundles. Scale bar=100 µm. e Representative traces of GCaMP6 signal from 1 Hz, 20 Hz, and acetylcholine (ACh, 10 mM) stimulated 2-week bundles. f Amplitude of electrically stimulated and ACh induced calcium transients increase with time of bundle culture. (*P < 0.05 vs. 1 week, #P < 0.05 vs. 20 Hz, Tukey–Kramer HSD test; n = 4–8 bundles per group at each time point). Data are presented as mean ± SEM
