Fig. 5
Localization of CuS-TRPV1 in high-fat-fed ApoE−/− mice. a US and PA imaging of the aortic arch in ApoE−/− mice intravenously received CuS-TRPV1 (upper panel) and CuS (lower panel). Yellow squares were used to indicate aortic arch location. b PA intensity of the aortic arch at different time-points. Data are shown as mean ± S.D. (n = 3), and analyzed by Student’s t-test. *P < 0.05 vs. pre. c Representative immunofluorescent micrographs of aortic roots sections demonstrating CuS-TRPV1 within atherosclerotic plaque at 2 h post-injection. From right, blue (DAPI), red (VSMC maker α-SMA or background control IgG), green (fluorescein-conjugated CuS-TRPV1 or CuS) and merge. The red line indicated the lesion border. The white square gave a view of the magnification of a region of lesion as indicated on the right (Scale bar = 75 μm, n = 3 mice per group). d and e Fluorescence intensity profiles of the regions enclosed by the white dotted lines
