Fig. 1 | Nature Communications

Fig. 1

From: Single-cell RNA-seq of rheumatoid arthritis synovial tissue using low-cost microfluidic instrumentation

Fig. 1

Microfluidic control instrument design and validation. a Picture of the microfluidic control instrument performing a Drop-seq run. b Top down views of multi-tiered instrument. Levels 1–4 contain assorted components for instrument operation. c 3D rendering of the instrument with levels corresponding to those in b. Components in light gray are 3D printed. d Microscope image screen capture directly from the instrument. Cells and barcoded microparticles are visualized easily on the screen. e Microscope image of droplets output from the instrument. Droplets and microparticles are detected via image analysis software as blue circles and green circles respectively. Inset: droplet diameter distribution histogram. f Microparticle loading distribution into droplets as measured via automated image analysis is consistent with Poisson loading. g Species mixing experiments using mouse (3T3) and human (HEK293) cells at total cell concentrations of 75 cells/µl (left) and 300 cells/µl (right)

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