Fig. 3
From: SWELL1 is a glucose sensor regulating β-cell excitability and systemic glycaemia
SWELL1 depletion reduces swell- and glucose-stimulated β-cell membrane depolarization. a β-cell membrane potential measured in current-clamp mode upon application of hypotonic solution (210 mOsm/kg) in WT (left) and Swell1 KO (right) murine primary β-cells. b Resting membrane potential (RMP) and c membrane depolarization rate in WT (n = 10) and Swell1 KO (n = 8) murine primary β-cells in response to hypotonic swelling. d β-cell membrane potential measured in current-clamp mode upon application of hypotonic solution (210 mOsm/kg) in WT (left) and SWELL1 KD (right) human primary β-cells. e RMP and f membrane depolarization rate in WT (n = 5) and SWELL1 KD (n = 10) human primary β-cells in response to hypotonic swelling. g Glucose (16.7 mM)-elicited membrane depolarization in isolated WT (left) and Swell1 KO murine β-cells. h RMP and i membrane depolarization rate in WT (n = 10) and Swell1 KO (n = 9) murine primary β-cells in response to 16.7 mM glucose stimulation from 1 mM basal glucose. All of the recordings above were performed at 35–37 °C in perforated patch configuration, current-clamp mode. Data are shown as mean ± s.e.m. *p < 0.05; **p < 0.01; ***p < 0.001, unpaired t-test. ns, not significant
