Table 1 Antibiotic susceptibility of mphI and two library mutants with expanded resistance to C3 cladinose macrolides expressed in E. coli BW25113 ΔbamBΔtolC

From: The evolution of substrate discrimination in macrolide antibiotic resistance enzymes

Antibiotic

Empty vector

mphI

azi_1.1 a

ery_1 b,c

Erythromycin

0.5

1

16

16

Clarithromycin

0.5

1

4

4

Azithromycin

0.063

0.25

2–4

2–4

Telithromycin

0.13

>16

>16

>16

Spiramycin

2

256

256

256

Tylosin

2

1024

1024

1024

Josamycin

2–4

>64

>64

>64

Kanamycin

1

1

1

1

  1. aMphIM40A/S111R/T166E/P211L
  2. bMphIM40A/S111G/T166E/P211L
  3. cMphI mutant Ery_1 was found to contain mutations in the promoter region, potentially altering expression and resistance phenotype. Ery_1 was therefore subcloned into the wild-type pGDP4 empty vector. MICs in this table are directly comparable.
Back to article page