Fig. 1

Identification of USP13 as a candidate MCL1 deubiquitinase. a The quantification of MCL1 protein levels upon knockdown of each DUB in HEK293T cells. 84 siRNA pools were individually transfected into HEK293T cells, endogenous MCL1 was detected by western blotting and protein bands were quantified using the Image J software. b Classification of the 84 human DUBs included in siRNA screen. c Dot plot of copy number alterations for 20 candidate MCL DUB genes in TCGA cancers. d Validation of the candidate MCL1 DUBs in HEK293T cells. Four different siRNAs targeting USP9X, OTUB2, or USP13 were transfected into HEK293T cells, and endogenous expression levels of indicated proteins were measured by western blot analysis