Fig. 6

Klf4 knockdown decreases Stau1 binding to target mRNAs and slows the rate of SMD. a NPCs infected with shScramble or shKlf4 lentivirus were treated with actinomycin D (5 μg/ml) for indicated times and mRNAs were prepared for RNA stability assays (n = 3). b RT-qPCR to assess the mRNA decay following Stau1 overexpression in NPCs transduced with shScramble or shKlf4 knockdown constructs. NPCs were treated with actinomycin D (5 μg/ml) for indicated times, and mRNAs were prepared for RNA stability assay (n = 3). c RT-qPCR to assess the mRNA decay at various time points following Ddx5/17 loss in NPCs overexpressing (OE) Klf4 (n = 3). d PAR-CLIP qPCR to assess Stau1 enrichment on target mRNAs in NPCs transduced with shKlf4 knockdown or shScramble constructs. Dlx1 (#6), Dlx2 (#6), and Tuj1 (#2) primers were used, and α-IgG was used as a negative control. Data are presented as mean ± SD. t tests were performed to calculate statistical significance (*P < 0.05, **P < 0.005, ***P < 0.0005). See also Supplementary Fig. 11a–d, 12c, and Supplementary Table 4