Fig. 5
From: Lsd1 regulates skeletal muscle regeneration and directs the fate of satellite cells
Lsd1 targets muscle-specific genes under myogenic but not under adipogenic conditions. a Heatmap obtained from RNA-seq analyses depicting differentially expressed genes (DEGs) in: wild-type C2C12 cells differentiated for 1 day in myogenic versus adipogenic medium (MYO/AD, first column), LSD1 overexpressing versus control C2C12 cells (OE/Ctrl, second column), or Lsd1 knockdown versus control C2C12 cells (KD/Ctrl, third column) differentiated for 1 day in adipogenic medium. b Pathway analysis showing significantly enriched networks for the set of 275 inversely regulated genes (IRGs). c Overlap of all 349 IRGs (pink) obtained from RNA-seq analyses and genes with Lsd1 promoter occupancy (dark gray) obtained from ChIP-seq analyses in C2C12 cells differentiated for 1 day in myogenic medium. d ChIP-seq tracks depicting Lsd1 chromatin occupancy at indicated promoters in wild-type C2C12 cells differentiated for 1 day in myogenic or adipogenic medium or in LSD1 overexpressing [LSD1(OE)] C2C12 cells differentiated for 1 day in adipogenic medium. Identified peaks are marked with bars. e ChIP-seq tracks depicting Lsd1 chromatin occupancy at the promoter of Glis1 in C2C12 cells differentiated for 1 day in myogenic or adipogenic medium. Identified peaks are marked with bars. (a, b: n = 3)
