Fig. 3
Genomic instability in mrc13A cells depends on transcription. a Deletion of the MSN2 and MSN4 transcription factors suppresses the high TAR levels of the mrc13A strain upon stress. Numbers are relative to the recombination levels in control conditions. b The mrc13Amsn2 msn4 strain displays fewer Rad52-YFP foci than the mrc13A strain upon stress. Experiments in a and b were performed as in Fig. 2. Data in a and b represent the mean and standard deviation of three independent experiments. Values that are significantly different (*p < 0.05, **p < 0.01, ***p < 0.005) by Student's t test are annotated. c DNA polymerase proceeds more slowly across the CTT1 gene upon osmostress in mrc13A cells when transcription is active. Schematic diagram of the ARS727 and the CTT1 loci. The association of Dpb2-HA at the indicated ARS727 and CTT1 sites (colored bars) was determined using ChIP. Graphs show the kinetics of Dpb2-HA binding after release in YPD (Con) or osmostress (NaCl). The red square highlights the relevant time points. Data represent the mean and standard deviation of three independent experiments
