Fig. 3

Loss of DOT1 suppresses the increased histone exchange at transcribed regions in set2Δ cells. a The ChIP-seq average plot of H4ac normalized by H3. The values for set2Δ (black), dot1Δset2Δ (magenta), and dot1Δ (blue) cells were calculated as the log₂ ratios of the mutant over wild type. The values of the y-axis indicate log₂ fold change level of H4ac/H3 ChIP-seq in mutants over wild-type cells, and the x-axis indicates the distance from transcription start sites (TSS) and transcription end sites (TES). The data set included all transcribed regions of yeast (number of genes, n = 6692), and were obtained from biological duplicates. b Distribution of H3 at the BLM10 (left) and POL2 (right) genes. The ChIP-seq data were obtained from biological duplicates. The y-axis indicates log₂ fold change of H3 in mutants over wild type. The H3 levels were normalized as log₂ (mutant/wild-type) values using the spike-in normalization method. The plus and minus values of the y-axis indicate the increased and decreased level of each mutant with respect to wild type. The gray boxes indicate the transcribed regions of BLM10 and POL2. The orange boxes indicate an enlarged view for BLM10 and POL2 loci. The y-axis values represent the values of zoom-up box. c A boxplot of histone H3 log₂ fold change values (normalized by the spike-in method). The y-axis indicates log₂ fold change of H3 in mutants over wild type. The values presented for set2Δ (black) and dot1Δset2Δ (magenta) are relative to the wild-type values at the transcribed regions of all yeast genes (number of genes, n = 6692). The shadowed box indicates negative values, indicating the decreased region in mutant with respect to wild-type. ***P-value <0.001 (Wilcoxon and Mann–Whitney tests). d A boxplot of histone H3 log₂ fold change values obtained for dot1Δ (blue) and Dot1(101–140Δ) (yellow) at the transcribed regions of all yeast genes (number of genes, n = 6692). The y-axis indicates log₂ fold change of H3 in mutants over wild type. The shadowed box indicates negative values, indicating the decreased region in mutant with respect to wild type