Fig. 3

Identification of host-directed compounds inhibiting Stm. a Screen of the LOPAC library in the HeLa-Stm infection model using Stm constitutively expressing stable DsRed, as in Fig. 2a. b Rescreen of the hit compounds from the HeLa-Stm screen that were superior to H-89 without affecting cell viability, displayed as in Fig. 2b. The bacterial load is expressed as a z-score in the utmost left panel and as a percentage of control value ± standard deviation in the middle panel to indicate the extent of bacterial inhibition. Compound abbreviations: T = trimethoprim, H = haloperidol, M = mibefradil, O = ofloxacin. c Overnight treatment of a Stm broth culture with the hit compounds at 10 µM. Gentamicin (50 μg/ml) was used as a positive control. The average bacterial density ± standard deviation of six replicates from a representative experiment out of three experiments is shown. The bacterial load is expressed as a percentage of the DMSO control value to indicate the extent of bacterial inhibition. Statistically significant difference compared to DMSO was tested using a one-way ANOVA (F_(5,30) = 4871; ***p value < 0.001). d Comparison of the Stm and Mtb primary screening data. Compounds that were superior to H-89 and subsequently confirmed in a rescreen are indicated in gray triangles. H = haloperidol