Fig. 1
Experiment design and hiPSC characterization. a Schematic: generation of neural progenitor cells (NPCs) from discordant twins’ hiPSCs following ZIKVBR infection and analysis. Silhouettes are courtesy of www.vecteezy.com (mother) and Yulia Ryabokon (babies). b, c Immunofluorescence for TRA-1-60 and OCT3/4 in hiPSCs. Scale bar, 20 μm. d MLPA analysis of subtelomeric imbalance chromosomal abnormalities in cultured hiPSCs cell lines using P070 and P036 MLPA Kits. e, f RT-qPCR analysis of hiPSCs for OCT3/4 and NANOG expression (mean ± SEM). g Representative discordant twins’ computed tomography (CT) scan images (a′, b′ [#10763-4]; e′, f′ [#10608-4]—non-affected babies; c′, d′ [#10763-1], g′, h′ [#10608-1]—affected babies). #10608 twins were 5 months old and #10763 were 15 months old when submitted to CT scan. White arrows show observed brain abnormalities (calcifications, ventriculomegaly, and cortical gyrus simplification). Scale bar, 3 cm. h Ventricular area analysis (cm2) (n = 4, two pairs of discordant twins). i Cortex thickness analysis (n = 4, two pairs of discordant twins). ***p < 0.001; **p < 0.01; mean ± SEM; Student’s t test
