Fig. 1
From: HTLV-1 Tax plugs and freezes UPF1 helicase leading to nonsense-mediated mRNA decay inhibition
HTLV-1 Tax protects reporter and endogenous mRNAs from NMD in mammalian cells. a RNA decay assays were carried out in HeLa cells. The stability of Gl-PTC and Gl-WT mRNA was analysed after RNA quantification by qRT-PCR. mRNA half-lives (t1/2 = ln(2)/λ with λ the time constant of the decay curves) are indicated in front of their respective conditions. b Same as a except that HeLa cells were co-transfected with HTLV-1 molecular clone. c RNA decay assays of Gl-PTC mRNA in the presence of WT HTLV-1 molecular clone (continuous line, diamonds), truncated ΔpX HTLV-1 molecular clone complemented with Tax protein (continuous line, triangle), truncated ΔpX HTLV-1 molecular clone alone (dashed line, square) and empty vector (dashed line, circle). d RNA decay assays examining the stability of endogenous GADD45α mRNA in the presence of an empty vector (dashed line, square), Tax (continuous line, cross) or a WT HTLV-1 molecular clone (continuous line, diamond; e), f Same as d with SMG5 and MAP3K14 endogenous mRNA. The values represented in each graph correspond to the mean of at least three biological replicates, and the error bars correspond to the SD. Half-lives were calculated for each replicate, and P values were calculated by performing a Student’s t-test (unpaired, two-tailed) ns: P > 0.05; *P < 0.05; **P < 0.01
