Fig. 1

Study schematic and steroid hormone receptor binding in male breast cancer. a A graphic visualization of the study design. The male silhouette was from Wikipedia (https://en.wikipedia.org/wiki/File:Male_Bathroom_Symbol.png). The female silhouette was from Wikipedia (https://commons.wikimedia.org/wiki/File:Toilet_women.svg), from a collection commissioned by the United States Department of Transportation, designed by AIGA http://www.aiga.org/content.cfm/symbolsigns, and converted into SVG by Wikipedia-user Lateiner. The image is used under a CC-BY 2.5 license. b Genome browser snapshot, depicting two known ERα bound regions with read counts for 2 random samples chosen for each factor. Genomic coordinates and read counts are indicated above. c Heatmaps depicting peak intensity in primary tumors for 30 ERα (blue), 7 FOXA1 (light green), 10 AR (red), 7 GR (black), 4 PR (purple), and 3 GATA3 (dark green) binding events (±5 kb from the peak center (triangle)). 5079 ERα sites were determined using the consensus ERα binding sites identified in at least 50% of patients (15 out of 30 samples). d Pie charts depicting genomic distributions for the consensus binding sites of each of the factors: AR (shared in 5 out of 10 samples), FOXA1 (3 out of 7 samples), PR (2 out of 4 samples), GR (3 out of 7 samples), and GATA3 (1 out of 3 samples). e Negative Z-score of the top 5 sequence motifs for binding sites of each factor depicted as a barplot. Colored (non-gray) bar represents the target factor’s sequence motif